化學(xué)交聯(lián)結(jié)合質(zhì)譜技術(shù)（chemical cross-linking coupled with mass spectrometry，CXMS）技術(shù)在近十年內(nèi)得到了蓬勃發(fā)展。被廣泛應(yīng)用于蛋白質(zhì)結(jié)構(gòu)鑒定和蛋白質(zhì)相互作用分析。目前CXMS技術(shù)在實(shí)際應(yīng)用存在一定的局限性：1、復(fù)雜樣品處理效率偏低；2、能夠提供的交聯(lián)信息有限。該技術(shù)發(fā)展的最大瓶頸已經(jīng)體現(xiàn)在有限的化學(xué)交聯(lián)劑選擇和交聯(lián)效率上。百靈威作為專(zhuān)業(yè)供應(yīng)商，提供新型化學(xué)交聯(lián)劑Leiker和d6-Leiker，具有可富集、可切斷、可定量的作用[1]。
■ Leiker具有高效的富集功能，比傳統(tǒng)交聯(lián)劑有更高的效率
Figure 1. Leiker(2138035) allowed near 100% enrichment of target peptides from a cross-linked ten-protein mixture diluted with increasing amounts of non-cross-linked E. coli lysates[1].
■ Leiker可以顯著提高所能鑒定到的交聯(lián)肽段數(shù)量
Figure 2. CXMS analyses of E. coli and C. elegans lysates. The best protein-protein interaction cluster extracted from the Leiker-identified or BS3(146482)-identified (Yang et al., 2012) [2]inter-links from E. coli whole-cell lysates[1].
■ Leiker可以進(jìn)行定量CXMS研究
Figure 3. Quantitative CXMS analysis of the L7Ae-RNA complex. Reciprocal labeling of RNA-free (F) and RNA-bound (B) L7Ae with [d0]/[d6]-Leiker(2322681)[1].
References
[1] Tan D, Li Q, Zhang M J, et al. Trifunctional cross-linker for mapping protein-protein interaction networks and comparing protein conformational states[J]. Elife Sciences, 2016, 5.
[2] Yang B, Wu Y J, Zhu M, et al. Identification of cross-linked peptides from complex samples.[J]. Nature Methods, 2012, 9(9):904-906.
■ 新型蛋白質(zhì)交聯(lián)劑
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營(yíng)銷(xiāo)編號(hào)
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產(chǎn)品名稱(chēng)
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中文名稱(chēng)
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包裝規(guī)格
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2138035
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Leiker, 95%
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賴氨酸特異性可富集型交聯(lián)劑
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5 mg
10 mg
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2322681
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d6-Leiker, 95%
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d6-賴氨酸特異性可富集型交聯(lián)劑
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5 mg
10 mg
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■ 其他交聯(lián)劑系列產(chǎn)品
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產(chǎn)品編號(hào)
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產(chǎn)品名稱(chēng)
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CAS
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包裝規(guī)格
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350636
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4-Benzoylbenzoic acid N-succinimidyl ester, 97%
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91990-88-4
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50 mg
250 mg
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146482
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Bis(sulfosuccinimidyl) suberate sodium salt, BS3, 90%
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82436-77-9
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25 mg
100 mg
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296329
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Di(N-succinimidyl) 3,3'-dithiodipropionate, DTSP, 97%
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57757-57-0
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1 g
5 g
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456454
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Di(N-succinimidyl) glutarate, DSG, 97%
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79642-50-5
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50 mg
250 mg
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253405
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Di(N-succinimidyl) suberate, DSS, 98%
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68528-80-3
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1 g
5 g
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1410146
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Disuccinimidyl tartrate, DST, 95%
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N/A
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5 mg
25 mg
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792543
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3,3'-dithiobispropionic acid bis-sulfosuccinimidyl ester, DTSSP, 90%
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81069-02-5
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25 mg
100 mg
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444282
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N-Succinimidyl iodoacetate, 95%
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39028-27-8
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100 mg
500 mg
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|
551313
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Succinimidyl 3-(2-Pyridyldithio)Propionate, SPDP, 97%
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68181-17-9
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100 mg
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■ 相關(guān)產(chǎn)品
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產(chǎn)品編號(hào)
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英文名稱(chēng)
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中文名稱(chēng)
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CAS
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649777
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Acrylamide/?Bis-?acrylamide Solution, 29:1, 40%
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丙烯酰胺/雙丙烯酰胺溶液
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N/A
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398539
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Aprotinin, 6000 U/mg
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抑肽酶
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9087-70-1
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|
3922801
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BCA Protein Assay Macro Kit
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蛋白質(zhì)定量試劑盒
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N/A
|
|
266630
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Benzamidine hydrochloride, 99%
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苯甲脒鹽酸鹽
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1670-14-0
|
|
1914018
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CAPS Buffer
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CAPS 緩沖液
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N/A
|
|
327499
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Coomassie Brilliant Blue G
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考馬斯亮藍(lán)G
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6104-58-1
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|
415951
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Dithiothreitol, 99%
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二硫蘇糖醇
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3483-12-3
|
|
1914020
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Phosphate Buffered Saline, 10×
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PBS緩沖液
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N/A
|
|
3910101
|
Protease Inhibitor Mix G
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Cocktail蛋白酶抑制劑G
|
N/A
|
|
3910201
|
Protease Inhibitor Mix M
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Cocktail蛋白酶抑制劑M
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N/A
|
|
1201851
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Protein Color Marker, M.W. 1,200 - 31,000 Da
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蛋白預(yù)染Marker
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N/A
|
|
1914019
|
SSC Buffer, 20×
|
SSC 緩沖液
|
N/A
|
|
1913953
|
Tris-Glycine-SDS Running Buffer, 10×
|
Tris-Glycine-SDS電泳緩沖液
|
N/A
|
|
954773
|
Trypsin, 2500 units/mg
|
胰蛋白酶
|
9002-07-7
|
■ 其他配套產(chǎn)品：